Macrochelid mites are known for their phoretic/facultatively parasitic behavior on fly hosts. A recent investigation into the host-attachment behavior of individual age-controlled mites revealed consistent behavioral differences- some mites attached to a different fly host repeatedly, and others never attached after multiple days of exposure. Multiple factors could contribute to these behavioral differences among individuals, including the presence/absence of endosymbiotic microbes. This study identified and compared the microbiomes of individual Macrochelid mites exhibiting host attachment and non-host attachment behavior.