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ViewAttendees 2

10-Minute Paper

Plant-Insect Ecosystems

P-IE: Microbial Control and Microbial Interactions

Evaluation of Chromobacterium Csp_P biopesticide in controlling key insect pests

Sunday, November 5, 2023
9:12 AM - 9:24 AM ET
Location: Gaylord National Resort & Convention Center, National Harbor 13
In-Person
Bacterial insecticidal proteins have long been utilized to combat insect infestations. The Chromobacterium species Panama (Betaproteobacteria: Neisseriaceae), referred to Csp_P was isolated from mosquito vectors Aedes aegypti midguts, which were collected in dengue-endemic areas in Panama. Csp_P has been demonstrated to have high insecticidal activity against the larvae of three mosquito vector species and to larvae of three Diabrotica species. The objective of the present study was to evaluate the susceptibility of multiple key insect pests including rice pests as well as some generalist pests. A Csp_P non-live air-dried preparation was generated by using the method called ‘nonlive_1’. To measure mortality, insects were subjected to a 7-day diet toxicity assay. Along with mortality, the lethal concentration (LC50) based on larval or adult survival data and the effective concentration (EC50) based on surviving larvae dry weight were calculated. Our preliminary results indicated that Csp_P could be an effective alternative to chemical pesticides in controlling these key insect pests.