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ViewAttendees 2

10-Minute Paper

Systematics, Evolution, and Biodiversity

SysEB: Evolution and Phylogenetics 3

Are Fleas truly Scorpionflies: Advances in bioinformatics facilitate a new phylogenetic hypothesis of Siphonapteran and Mecopteran relationships

Wednesday, November 8, 2023
10:00 AM – 10:12 AM ET
Location: Gaylord National Resort & Convention Center, National Harbor 10
In-Person
We present SAPPHYRE, an all-in-one dataset preparation package. Using data from Dietz et al. 2023 we compare the output of SAPPHYRE against PHYLUCE, HybPiper, Orthograph, BWA mapping, IBA, and the combined approaches of Orthograph + Phyluce and Orthograph + Phyluce + Trinity. Taken against the medians of all seven approaches, SAPPHYRE, compared to the next best approach, returns 2.17x longer gapped alignments, 1.5x additional gapped parsimoniously informative sites, 1.57x longer un-gapped alignments and 1.54x additional un-gapped parsimoniously informative sites. Overall, SAPPHYRE placed first in 41 of 42 of these measured metrics and second in one. Molecular phylogenetics inconsistently recovers the position of Siphonaptera (fleas) with regards to Mecoptera (scorpionflies). Using SAPPHYRE, we mine 3643 USCOs found across Holometabola, to assemble a dataset constructed from twelve fleas and fourteen scorpionflies. We analyze the resulting amino-acid and 1+2 position nucleotide datasets using IQTREE2 and reassess these relationships.