Morphology and molecular based phylogeny of Korean Chrysis ignita group (Hymenoptera: Chrysididae)

The ignita group is the largest and taxonomically challenging group within the genus Chrysis; they exhibit merely similar morphology and adopt a parasitic lifestyle. The morphological characteristics might be contributed to misidentification and inconsistent interpretations of ignita group species. Only 12 species of ignita group, including misidentified species, were recorded in Korean Peninsula. Here, 33 samples were collected and used for morphologically, molecular identification. Universal primers (COI) and newly designed primers that encoded partial mitochondrial genome nearby D-loop regions (between nd4 and 16S rRNA) were used. In addition, the complete mitochondrial genome of representative, Chrysis principalis length was 15,917bp. Compared COI sequences deposited in GenBank based on the sequence homology (100% coverage and identity), we found six species, C. fulgida, C. impressa, C. angustula, C. longula, C. ignita and C. subcoriacea among all the tested samples. So far, our knowledge, Chrysis impressa Schenck, 1856, Chrysis angustula Schenck, 1856 and Chrysis subcoriacea Linsenmaier, 1959 were recorded for the first time in Korea.