Mealybugs (Pseudococcidae) are among the most injurious pests of food, fiber, and ornamental crops worldwide. Their wide host range means that wild hosts in unmanaged areas are often negatively impacted as well. South Florida is home to many native and invasive mealybugs, but their current prevalence and distribution is unknown. Their natural enemies are similarly undescribed. The purpose of this study is to survey mealybugs and their natural enemies in south Florida’s natural and managed habitats. To this end, 10 natural areas and 10 managed habitats were selected for study. At each site, three 10x10m representative sampling sites were delineated. Within each of these areas, the plant and mealybug species were inventoried. When found, mealybugs were collected and maintained in the laboratory to determine if they were parasitized. Other natural enemies were identified and recorded when present. This study provides important baseline data on native and introduced mealybugs and their natural enemies in Florida. Ultimately, this information can be used to inform future classical biological control and conservation efforts.